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. 2008 May 9;14(7-8):443–450. doi: 10.2119/2008-00015.Qiang

Figure 5.

Figure 5

Stability of purified human AMBP-1. Monolayer of rat peritoneal macrophages was incubated with or without LPS (10 ng/mL) in the absence or presence of human AM (200 nM)/AMBP-1 (100 nM), purified or commercially available. The isolated and purified human AMBP-1 was well preserved when stored at 45°C for 5 d in solution or 100°C for 1 h in powder. Purified human AMBP-1 solution stored at −20°C was used as a positive control. The levels of TNF in the culture medium were determined by ELISA and expressed as mean ± SEM of three independent experiments (in duplicate) and compared by one-way ANOVA and Student-Newman-Keuls method. *P < 0.05 compared with None; #P < 0.05 compared with LPS alone.