Table 2.
Ct-values of CT- and (+/-) LGV- positive specimens tested in real-time PCRs in dependence on multiplexing
| Sample | Specimen | CT (+/-IK_C)a | LGV (+/-IK_C)a | IK_C | L1 (+/-L2) | L2 (+/- L1) | L3 (+/-GAPDH) |
| L1 | DNA | 26/26 | 28/27 | 38 | 28/28 | 42/- | -/- |
| L2 | DNA | 27/26 | 27/27 | 42 | -/- | 28/30 | -/- |
| L3 (dilut.) | DNA | 34/34 | 33/35 | 32 | -/- | -/- | 34/35 |
| 04/256 | Ocular | 34/36 | -/- | 37 | 42/- | -/- | 42/- |
| 04/270 | Urethral | -/- | -/- | 36 | 40/- | -/- | -/39 |
| 04/1180 | Rectal | 26/26 | -/- | 36 | -/- | -/- | -/- |
| 06/3013 | Rectal | 35/36 | -/- | 36 | -/- | -/- | -/39 |
| 04/234 | Rectal | 23/23 | 25/24 | - | -/- | 26/28 | -/- |
| 04/227 | Rectal | 32/31 | 34/33 | 34 | -/- | 35/36 | -/- |
| 04/261 | Rectal | 31/30 | 34/33 | - | -/- | 38/37 | -/- |
| 04/985 | Rectal | 31/30 | 34/32 | 34 | -/- | 34/32 | 40/- |
| 05/697 | Rectal | 29/29 | 31/31 | 36 | -/- | 33/33 | 36/35 |
| 06/567 | Urethral | nt/34 | nt/34 | 36 | -/- | 36/37 | -/- |
| 06/1507 | Rectal | 28/28 | 31/31 | 35 | -/- | 31/34 | -/39 |
a CT/LGV-real-time PCR with (+) and without (-) addition of the internal control plasmid IK_C