Figure 5.

Inhibition of hTLR2 promoter activity by DNA methylation. SssI methylase-treated or untreated pGL3-T2P (-120) was co-transfected into HeLa cells with the pcDNA3.1 or the SP1 plasmid. HeLa cells were also co-transfected with pGL3-null and pcDNA3.1 as control. Luciferase activity is expressed as the percent activity of the pGL3-T2P (-120) plasmid in pcDNA3.1-co-transfected, methylase-untreated cells. Data are presented as the mean ± S.E.M. from three independent experiments performed in triplicate. *p < 0.0001, assessed by Student's t-test.