Table 1.
Oligonucleotides used for this study
| Primers used for the generation of TLR2 promoter/reporter constructs | ||
| pGL3-T2P(-309) | 5'-GCCTCTAGCGTCTCGATTCGC-3' | 5'-CTGGGAGAACTCCGAGCAGTC-3' |
| pGL3-T2P(-194) | 5'-CTGTCGCAGCCTAGCTCACGG-3' | 5'-CTGGGAGAACTCCGAGCAGTC-3' |
| pGL3-T2P(-120) | 5'-CCTGCCTGGAACTCAGCGCG-3' | 5'-CTGGGAGAACTCCGAGCAGTC-3' |
| pGL3-T2P(-60) | 5'-TGCCCCGTGGAAGGGGCGGTT-3' | 5'-CTGGGAGAACTCCGAGCAGTC-3' |
| pGL3-T2P(-12) | 5'-CGCGCGGACTTTCCCTTTTGC-3' | 5'-CTGGGAGAACTCCGAGCAGTC-3' |
| Primers used for the generation of mutant TLR2 promoter/reporter constructsa | ||
| pGL3-T2P(-120) SPm | 5'-CCCGTGGAAGGTTAGGTTCCCGCACCCCAG-3' | 5'-CTGGGGTGCGGGAACCTAACCTTCCACGGG-3' |
| pGL3-T2P(-120)/(-60) ETSm | 5'-GCGCACGTGCCCCGACGAAGGGGCGGTTCC-3' | 5'-GGAACCGCCCCTTCGTCGGGGCACGTGCGC-3' |
| Primers used for semi-quantitative RT-PCR | ||
| SP1 | 5'-TGTGAATGCTGCTCAACTCTCC-3' | 5'- CATGTATTCCATCACCACCAG -3' |
| SP3 | 5'-AACCTGATCCTGAAGAGTGGC-3' | 5'-TGGCGGAAGTATTAACAGTTCC-3' |
| Oligonucleotides used for EMSAb | ||
| T2P(-64/-31) | 5'-GTGCCCCGTGGAAGGGGCGGTTCCCGCACCCCAG-3' | 5'-CTGGGGTGCGGGAACCGCCCCTTCCACGGGGCAC-3' |
| T2P(-64/-31) SPm | 5'-GTGCCCCGTGGAAGGTTAGGTTCCCGCACCCCAG-3' | 5'-CTGGGGTGCGGGAACCTAACCTTCCACGGGGCAC-3' |
| T2P(-64/-31)-monoMe | 5'-GTGCCCCGTGGAAGGGGCGGTTCCCGCACCCCAG-3' | 5'-CTGGGGTGCGGGAAC/M-C/GCCCCTTCCACGGGGCAC-3' |
| T2P(-64/-31)-triMe | 5'-GTGCCCCGTGGAAGGGGCGGTTCCCGCACCCCAG-3' | 5'-CTGGGGTG/M-C/GGGAAC/M-C/GCCCCTTCCA/M-C/GGGGCAC-3' |
a Mutations introduced in the oligonucleotides are shown in italic.
b Putative SP1 binding sequences are underlined. Mutations introduced in the oligonucleotides are shown in italic. M-C represents methylated cytosine.