Table 1.
Overview of SupT1 clones with integrated HIV-rtTA provirus
Clone | HIV-rtTA gDNA PCR | HIV-1 resistant1 | Dox-induced | Number detected proviruses | |||
HIV-rtTA replication2 | Intracellular CA-p243 | ||||||
(24 h) | (48 h) | M.F.I.5 | |||||
B5 | + | - | - | 0.01 | 0.04 | - | -4 |
B8 | + | - | - | 0.38 | 0.34 | 62 | - |
C2 | + | + | - | 0.04 | 0.00 | - | 1 |
C4 | + | + | - | 0.01 | 0.00 | - | - |
C7 | + | + | - | 0.00 | 0.00 | - | 2 |
D2 | + | + | - | 0.01 | 0.06 | - | 1 |
D10 | + | - | - | 0.01 | 0.02 | - | - |
D11 | + | + | - | 0.02 | 0.01 | - | 1 |
E4 | + | + | - | 0.00 | 0.01 | - | 2 |
F3 | + | - | - | 0.00 | 0.01 | - | - |
H7 | + | + | - | 0.00 | 0.00 | - | 3 |
B4 | + | + | + | 0.38 | 0.50 | 70 | 1 |
B6 | + | + | + | 0.22 | 0.19 | 78 | 5 |
B9 | + | + | + | 1.34 | 1.66 | 69 | 3 |
C5 | + | + | + | 0.72 | 0.82 | 106 | 3 |
C11 | + | + | + | 0.01 | 0.04 | - | 3 |
D4 | + | + | + | 0.07 | 0.02 | - | 4 |
D6 | + | + | + | 0.07 | 0.02 | - | 1 |
E7 | + | + | + | 0.43 | 0.59 | 100 | - |
E11 | + | + | + | 0.05 | 0.02 | - | - |
F7 | + | + | + | 10.52 | 11.54 | 49 | 2 |
F8 | + | + | + | 1.31 | 2.16 | 82 | 1 |
F9 | + | - | + | 0.07 | 0.02 | - | 1 |
G2 | + | + | + | 0.02 | 0.01 | - | 1 |
1 Wild-type HIV-1 LAI superinfection
2 HIV-rtTA replication scored by syncytia formation and extracellular CA-p24 production
3 Percentage CA-p24 positive cells
4 -; not detected
5 M.F.I. is the mean fluorescence intensity of the CA-p24 positive cells (48 h dox)