Table 3.
Real-time qPCR primers used in the study
| Genes | Primers | GenBank |
| ISAV segment 6 a | 5-AGGCCAAAAACGGAAATGGA-3 | AF220607 |
| 5-CCGTCAGTGCAGTCATTGGTT-3 | ||
| 18S rRNA b | 5-GCCCTATCAACTTTCGATGGTAC-3 | AJ427629 |
| 5-TTTGGATGTGGTAGCCGTTTCTC-3 | ||
| Galectin-3 binding prot c | 5-CCAGACCAACAGTGTTCACTTCAGC-3 | BX079375 |
| 5-ACGTGAAAGACATACCTGCCCTCAC-3 | ||
| Galectin-like 1 c | 5-CAGCAACCCTTCTTCAATCCGAGA-3 | CA344100 |
| 5-TCTCCCTGTCACAGTGATGGTCTTC-3 | ||
| Tripartite motif/ring finger protein c | 5-TGTTCTGTTGCTCCGTCTGTCTGGA-3 | CA376536 |
| 5-TTCAGCCAGCACGGTGTTTCTCTTC-3 | ||
| Tyr-protein kinase FRK c | 5-TAGACATGGCACCATGGACCCTC-3 | CA349577 |
| 5-GGGTTCTTCAGTGCAGACAGCCA-3 | ||
| STAT1 c | 5-GAACATGGAGGAGTCCAATGGAAGC-3 | CA343225 |
| 5-GGACCCTCATTTGATCTGTTGCCT-3 | ||
| CCAAT/enhancer binding protein beta c | 5-TACGTCCTGGGCTATCCTGAACTGC-3 | CA348284 |
| 5-CCAGACGAACCGTTGTTGTCCA-3 | ||
| Beta-2-microglobulin c | 5-TCGTTGTACTTGTGCTCATTTACAGC-3 | AF180478 |
| 5-CAGGGTATTCTTATCTCCAAAGTTGC-3 | ||
| IgM heavy chain (CH) d | 5-ATACGGTGACCCTGACTTGCTACGT-3 | S48652 |
| 5-TTCTCTCCACCGGCTCATCATCA-3 | ||
| IgZ CH-like d | 5-AGCACCAGGGACAAACCACCAT-3 | CA372094 |
| 5-TTCACACTCGGTGGGTTCAGAGTC-3 | ||
| IgD CH d | 5-TCTTCAGGAGCTGAGGACAGATGGA-3 | AF278717 |
| 5-AAACCCATCCACCTTCCAGCTGA-3 | ||
| CD4 d | 5-TGCATTGTTCCTCTCTTCCACAGC-3 | AY973028 |
| 5-CCGTCCCAAGGTACCATAGTACCAA-3 | ||
| TGF-beta d | 5-AATCGGAGAGTTGCTGTGTGCGA-3 | EU082211+ |
| 5-GGGTTGTGGTGCTTATACAGAGCCA-3 | AJ007836 | |
| CD8-alpha d | 5-CGTCTACAGCTGTGCATCAATCAA-3 | AY693391 |
| 5-GGCTGTGGTCATTGGTGTAGTC-3 | ||
| Interferon-gamma d | 5-TTCAGGAGACCCAGAAACACTAC-3 | AJ841811 |
| 5-TAATGAACTCGGACAGAGCCTTC-3 | ||
| GRB2-related adaptor protein 2 e | 5-TGACTTTACTGCCACTGCTGAGGAC-3 | CA353121 |
| 5-CAGTCATCATTGGTGCCCAAGATC-3 | ||
| Annexin A1-1 e | 5-CTCCAGGAAATTGAACACCGCGA-3 | CA364941 |
| 5-AAGGCTGCGATGAAGGACATGGT-3 | ||
| 5-lipoxygenase activating protein e | 5-TCTGAGTCATGCTGTCCGTAGTGGT-3 | CA369467 |
| 5-CCTCCCTCTCTACCTTCGTTGCAAA-3 | ||
| Regulator of G-protein signaling 1-1 e | 5-GACTCCTAACCTCCAATGCTTCGAC-3 | CA383094 |
| 5-CGAATCTCTCTCCATCAGCCCATA-3 | ||
| Cytochrome P450 2K4-1 e | 5-TTCACCCTCCACCCTTCACCTC-3 | EV384586 |
| 5-ATCTCAGACCCGGCTCACAGCA-3 | ||
| Galectin-9 e | 5-TCGCTGATTGTGAATGGTGCTCAC-3 | CX035552 |
| 5-CAGGGTTGGAGAAGGCAATGGATT-3 | ||
| Heat shock protein HSP 90-beta-2e | 5-GAACCTCTGCAAGCTCATGAAGGA-3 | BX074486 |
| 5-ACCAGCCTGTTTGACACAGTCACCT-3 | ||
| Cathepsin S e | 5-CGAAGGGAGGTCTGGGAGAGGAAT-3 | CA355014 |
| 5-GCCCAGGTCATAGGTGTGCATGTC-3 |
a Viral RNA quantification. b Reference gene. c Microarray confirmation and EM-induced genes, Fig. 4. d Adaptive immune responses all stages, Fig. 6. e Development of class prediction, Fig. 7.