Figure 2:

mRNA expression for TGFβ superfamily members by decidualized and non-decidualized HESC.

HESC were either non-decidualized or decidualized with cAMP for 7 days. Total RNA was extracted from cells for RT–PCR. Representative products demonstrating Activin βA, Activin βB, BMP2, BMP4, BMP7, GDF5, GDF8, GDF11, TGFβ1, TGFβ2, TGFβ3 and Nodal mRNA are shown in non-decidualized (ND) and cAMP-decidualized (D) HESC; +ve=positive control; −RT=negative control. Data shown is from a single experiment, which is representative of 2 independent culture experiments.