Figure 3:

Activin-M108A and SB43 154 inhibitors significantly decrease HESC decidualization.

Confluent stromal cells were cultured for 4–5 days with cAMP, with medium changes every 2–3 days. (A) M108A was added at doses 0.39, 1.5, 6.25 and 25 nM to cAMP-treated cultures. (B) SB431542 was added at doses 1.25, 2.5, 5 and 10 µM. Controls (not decidualized; ND) and cAMP (decidualized; D). PRL secretion from cells was used as a marker of decidualization, and values were corrected for total protein. All data (mean ± SEM) is expressed as % change from control (100%). Results are combined data from triplicate wells of five independent experiments for each inhibitor. *P < 0.05 and **P < 0.01 compared with cAMP alone.