Figure 5:

Effect of Meis1 expression on mouse litter size.

Litter size, as determined by counting the number of implantation sites per uterus examined, at Day 9 of pregnancy. Mice were transfected 1 day after detection of a vaginal plug with pTER/Meis1, control pTER, pcDNA4/MEIS1 or empty pcDNA4. (a) Litter size was reduced by 40% in the group of mice transfected with pTER/Meis1 siRNA compared with pTER controls. This difference was statistically significant according to a two-tailed unpaired t-test (P = 0.00001). (b) Litter size at Day 9 of gestation was not affected by transfection with pcDNA4/MEIS1 compared with pcDNA4-transfected controls (P = 0.08). The experiment was repeated three times, with similar results