Table 3.
Comparison between model predictions and experimental data for acidic pH at 48 h of growth
| Metabolite | WT | Experimental data (pmol/pmol Pi) |
Model predictions (pmol/pmol Pi) |
||
|---|---|---|---|---|---|
| GAL7:IPC1 | Δisc1 | GAL7:IPC1 | Δisc1 | ||
| Phytoceramide C26 (X9) | 222.5 | 523.3 | 10 | 465.08 | 9.37 |
| Phytoceramide C18 (X11) | 215.3 | 114.6 | 235 | 179.65 | 173.80 |
| ATP (X17)a | 82.48 | 80.36 | 16.92* | 82.48 | 29.17 |
apmol/μg protein.
*P<0.05, Δisc1 versus WT.
Phytoceramide C26 level is increased when Ipc1 is downregulated in the GAL7:IPC1-glucose strain compared to the control WT strains at pH 4. Values are concentrations in pmol phytoceramide C18 or C26/pmol phosphate (Pi). Results shown are the means of three independent experiments. ATP is significantly lower in the Δisc1 mutant compared to the control strain (WT) at pH 4.