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. Author manuscript; available in PMC: 2009 May 1.
Published in final edited form as: Biochem Pharmacol. 2008 Feb 7;75(9):1858–1867. doi: 10.1016/j.bcp.2008.01.017

Figure 3. DIM inhibits hypoxia-induced increased transcription of endogenous HIF-1-responsive genes.

Figure 3

MDA-MB-231 (A) and HepG2 (B) cells grown to near confluent density in 6-well plates were given the indicated concentrations of DIM in serum free medium and incubated in normoxic or hypoxic conditions for 24 hours. Total RNA was isolated and levels of mRNA of HIF-1 responsive genes were measured by semi-quantitative RT-PCR. Representative blots are shown. Experiments were reproduced at least three times with identical results.