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. Author manuscript; available in PMC: 2009 May 1.
Published in final edited form as: Biochem Pharmacol. 2008 Feb 7;75(9):1858–1867. doi: 10.1016/j.bcp.2008.01.017

Figure 6. Effect of DIM on intracellular oxygen availability.

Figure 6

Two days after transfection with the expression vector encoding Renilla luciferase, cells were trypsinized and resuspended in serum-free DMEM that had been pre-equilibrated with 1% O2 for two hours. The treatments were with DIM and the NO donor, NOC12, as the positive control, and the luciferase activity assays were all done in an hypoxic glove box filled with 1% O2/5% CO2 at 37 °C. Results shown are the average +/− SD of at least three replicates.