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. Author manuscript; available in PMC: 2009 May 1.
Published in final edited form as: J Nutr Biochem. 2007 Aug 17;19(5):336–344. doi: 10.1016/j.jnutbio.2007.05.004

Fig. 2.

Fig. 2

DIM induces ROS production in peritoneal macrophage cultures. (A) Peritoneal macrophages (5 × 105 per well) were cultured with CM-H2DCFDA with vehicle DMSO vehicle, DIM (20 μM) or 100 ng/mL of LPS for 2 h at 37 °C. Cells were detached by subjected to FACS analysis (B). Cultures were incubated with vehicle, DIM (10–40 μM) or LPS for 2 h, 6 h or 24 h. Cells were detached by trypsin/EDTA and analyzed by FACS. ROS production is presented as fold induction over the DMSO treatment after subtraction the fluorescence intensity of background. Results are presented as the mean ± SD (n=3). Asterisks indicate significant difference between DMSO-treated and DIM-treated/LPS-treated samples (P<0.05).