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Effect of Zinc on the Catalytic Life Span of Wild-Type CcO Containing Subunit III and CcO Lacking Subunit III
| average number of catalytic cycles required to inactivate the enzymea | ||
|---|---|---|
| addition | WT III(+) | WT III(−) |
| none | ∼1.6 × 106 | (6.2 ± 0.9) × 103 |
| 400 μM ZnSO4 | (105 ± 4) × 103 | (8.3 ± 0.4) × 103 |
No cholic acid, arachidonic acid, or soybean phospholipid was added to the assays. One catalytic cycle = 1O2 + 4e− + 4H+ → 2H2O.
