Figure 2.

Single channel record of αβγ-rENaC incorporated into a planar lipid bilayer membrane. The top trace was obtained under symmetrical ionic conditions with no hydrostatic or osmotic gradients present. The middle trace was obtained after 1 ml of solution was removed from the cis compartment. The bottom trace was recorded subsequent to the addition of 50 mOsm/kg sucrose to the trans compartment. This experiment is representative of seven such protocols.