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. 1997 Jul 8;94(14):7661–7666. doi: 10.1073/pnas.94.14.7661

Figure 4.

Figure 4

Genetic or pharmacological inhibition of p56lck prevents inactivation of the n-K+ channel by synthetic ceramides. The figure shows alterations of peak currents in percent of control peak currents (at +40 mV) 20 min after addition of 10 μM C6- or C2-ceramide or 10 μM dihydro-C2-ceramide in Jurkat cells, in p56lck-deficient JCaM1.6 cells, in p56lck-reconstituted JCaM1.6 cells, or in herbimycin A-pretreated Jurkat cells. Differences are significant (two tailed t tests) between the groups Jurkat + C6/C2-ceramide and Jurkat + dihydro-C2-ceramide and between Jurkat + C6-ceramide and JCaM1.6 + C6-ceramide or Jurkat + herbimycin A + C6-ceramide, respectively. The differences are also significant between JCaM1.6 + C6-ceramide and JCaM1.6/Lck+ + C6-ceramide. No significant difference could be detected among the groups Jurkat + C6-ceramide, Jurkat + C2-ceramide, and JCaM1.6/Lck+ + C6-ceramide.