Figure 9.

EPO-induced 24p3 lipocalin mRNA expression in ER343/401-S3 cells in the presence or absence of the protein synthesis inhibitor cyclo-heximide (CHX). ER343/401-S3 cells (Fig. 4) were cultured medium containing IL-3, then washed and transferred to medium containing 5 μg/L of CHX, 2 units/mL of EPO, or both EPO + CHX. Cells were harvested at the indicated times, and their total RNA extracted for Northern blot analysis, carried out as described in Figure 1.