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. Author manuscript; available in PMC: 2008 May 20.
Published in final edited form as: J Biol Chem. 2002 Mar 11;277(21):19001–19007. doi: 10.1074/jbc.M112271200

Fig. 2. G-CSF-responsive Stat3 signaling is abrogated in the presence of Stat3-DN.

Fig. 2

32D.G-CSFR and 32D.G-CSFR + Stat3-DN cells were maintained in WEHI-containing medium (W) or were washed extensively and then stimulated with medium containing 25 ng/ml G-CSF (G) for 2, 4, 6, or 12 h, as indicated. Total RNA was isolated and subjected to ribonuclease protection assays with probes specific for murine SOCS3 and GAPDH. SOCS3 mRNA protections are shown. GAPDH mRNA levels were equivalent in all samples (not shown).