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. 1999 Nov 9;96(23):12997–13002. doi: 10.1073/pnas.96.23.12997

Figure 2.

Figure 2

(A) Schematic presentation of screening and decoding of the combinatorial library. TAR RNA was labeled with a red dye, disperse red, at its 5′ end by chemical synthesis and incubated with the trimer library as described in the text. (B) A portion of the beads in the library after incubating with red dye-labeled TAR RNA. The dark bead in the center was identified as ligand 1. (C) The equilibrium interaction between dye-labeled TAR RNA and a tripeptide tethered to beads. A suspension of beads containing ligand 1 in TK buffer (400 μl) was incubated with dye-labeled TAR RNA (1 μM) at 4oC for 5 h. Beads were stained red upon TAR RNA binding (Left). Red beads became colorless when excess of unlabeled TAR RNA (Middle), or ligand 1 (Right) was added, indicating the displacement of red-TAR RNA from the beads.