Table 2.
| Peak | Compound | GU | % Glycan cores after
SFGF digest
|
ΔPrP27-30 %, +/− bisect | |||
|---|---|---|---|---|---|---|---|
| PrPC, Fig. 2Av | PrP 27–30, Fig. 2Bv | ||||||
| Total | +/− bisect | Total | +/− bisect | ||||
| A | A2G0 | 5.53 | 15.4 | 30 | 13.1 | 35 | +5 |
| J | A2G0B | 5.85 | 36.3 | 70 | 24.1 | 65 | −5 |
| B | A3G0 | 5.94 | 8.1 | 25 | 14.1 | 39 | +14 |
| K | A3G0B | 6.18 | 23.7 | 75 | 22.2 | 61 | −14 |
| F | A4G0 | 6.57 | 9.4 | 57 | 16.7 | 63 | +6 |
| L | A4G0B | 6.81 | 7.1 | 43 | 9.8 | 37 | −6 |
These were assigned from the elution positions of standard glycans and previously determined values for the addition of monosaccharides to glycan cores. Arthrobacter ureafaciens α2-6 > 3 sialidase (S) + almond meal α1-3/4 fucosidase (F) + bovine testes β1-3/4 galactosidase (G) + bovine kidney α1-6 fucosidase (F).