Table 2.
Nucleoside triphosphate specificity of phosphoryl donors for the phosphorylation of F-OH, F-P, GG-OH, and GG-P by tobacco microsomes
| Nucleotide added, 100 μM | % [3H]F-OH converted to
|
% [3H]GG-OH converted to
|
||
|---|---|---|---|---|
| [3H]F-P | [3H]F-P-P | [3H]GG-P | [3H]GG-P-P | |
| None | <0.3 | <0.3 | <0.3 | <0.3 |
| CTP | 18.2 | 22.1 | 37.4 | 20 |
| UTP | 13.8 | <0.3 | 66.0 | 2.3 |
| GTP | 13.2 | <0.3 | 50.1 | <0.3 |
| ATP | <0.3 | <0.3 | 12.8 | <0.3 |
The percent conversion of [3H]F-OH or [3H]GG-OH to the corresponding monophosphorylated and pyrophosphorylated forms was determined as described in Materials and Methods.