Figure 3.

Heterogeneous silencing suppression caused by highly diverse isolates. (A) GUS histochemical staining analysis of gus-silenced transgenic L10 leaves harvested at 35 dpi, controls correspond to non-inoculated (NI) and buffer-inoculated (BI) leaves from transgenic gus-expressing L4 and gus-silenced L10. In boxes, isolates belonging to the same serogroup (S, Figure 2) are compared. (B) GUS activity is measured by fluorimetry and is expressed as a percentage of maximal activity measured in Tz3 inoculated samples. (C) ELISA-based measurement of virus content and western blot analysis of P1 protein.