Figure 6.

TFIIS Recovery Assay. The TFIIS mammalian expression vector pCMV6-XL5 was purchased from Origene. MCF7 cells were co-transfected with 89, 107 and 125 ng of the TFIIS:pCMV6-XL5 plasmid and 0.1, 0.5, 1.0, 5.0 nM TFIIS siRNA. In addition, 178 ng of a control pCMV6-XL5 plasmid with no TFIIS insert was transfected with 0.1, 0.5, 1.0 and 5.0 nM TFIIS siRNA. For control, 100% proliferation was set as proliferation of cells treated with vehicle + plasmid alone (without siRNA) at the relevant concentrations. Data points represent mean and standard error. Cell proliferation was determined 72 hours after initial transfection of siRNA and expression vector.