Figure 7.

Relative protein levels of TFIIS, c-myc and p53 were determined in MCF7 and MCF10A cells. Protein values were determined for TFIIS siRNA #2 (Table 1) relative to GAPDH protein levels (TFIIS, c-myc or P53/GAPDH) and normalized with protein levels similarly determined from control S-siRNA treated cells. siRNA concentrations from 0.1 to 5.0 nM and standard error are indicated for each siRNA concentration. Data points represent mean and standard error and the assay performed 72 hours after initial siRNA transfection.