TABLE 1.

Nuclear tRNA genes and cytosolic codon usage in Chlamydomonas reinhardtii

Amino acids	Anticodonsa	Codonsb	Gene nos.a	% cytoc	Probesd
Ala	AGC	GCU–GCC	13	7.24	Al
	CGC	GCG	6 + 4	4.29	A1–A2
	UGC	GCA	3 + 1	1.06	A1–A2
Arg	CCG	CGG	3	1.1	Ri
	ACG	CGU–CGC	11	4.04	R2
	CCU	AGG	2	0.27	R3
	UCG	CGA	1	0.19	R4
	UCU	AGA	3	0.07	R5
Asn	GGU	AAU–AAC	7	3.09	N
Asp	GUC	GAU–GAC	11	4.83	D
Cys	GCA	UGU–UGC	7	1.39	C
Gln	UUG	CAA	1	0.43	Qi
	CUG	CAG	6	3.6	Q2
Glu	UUC	GAA	1	0.28	El
	CUC	GAG	13	5.41	E2
Gly	GCC	GGU–GGC	17	7.27	Gi
	UCC	GGA	1	0.5	G2
	CCC	GGG	1	0.94	G3
His	GUG	CAC	5	1.9	H
Ile	AAU	AUU–AUC	7	3.49	Ii
	UAU	AUA	1	0.1	12
Leu	CAG	CUG	10	6.48	Li
	CAA	UUG	2	0.39	L2
	UAA	UUA	1	0.06	L3
	UAG	CUA	1	0.26	L4
	AAG	CUU–CUC	3	1.73	L5
Lys	CUU	AAG	ii	4.42	Ki
	UUU	AAA	1	0.24	K2
Mete	CAU	AUG i	8	0.21	Mi
	CAU	AUG e	6	2.38	Me
Phe	GAA	UUU–UUC	9	3.24	F
SeC	UCA	UGA	1	ND	SeC
Pro	AGG	CCU–CCC	13	3.65	P
	CGG	CCG	6	2.02	P
	UGG	CCA	1	0.52	P
Ser	AGA	UCU–UCC	5	2.09	Si
	UGA	UCA	1	0.32	S2
	CGA	UCG	5	1.63	S3
	GCU	AGC–AGU	8	2.52	S4
Thr	AGU	ACU–ACC	6	3.35	Ti
	CGU	ACG	3	1.55	T2
	UGU	ACA	2	0.41	T3
Trp	CCA	UGG	5	1.31	W
Tyr	GAC	UAU–UAC	8	2.56	Y
Val	AAC	GUU–GUC	7	2.1	Vi
	CAC	GUG	10	4.61	V2
	UAC	GUA	1	0.21	V3

^aEach tRNA isoacceptor family is identified by its anticodon and the number of corresponding genes is given according to Merchant et al. (2007). For tRNA^AlaCGC and tRNA^AlaUGC, the two gene numbers refer to the two types of sequences (groups I and II, see Figure 2) identified for each isoacceptor.

^bMinimal potential codon recognition pattern of tRNA isoacceptors listed according to the anticodons in the previous column.

^cCytosolic (% cyto) codon usage as obtained from http://www.kazusa.or.jp/codon/.

^dNames of probes are according to supplemental Table 2. The plastidial tRNA^IleGAT gene inserted into the nuclear genome is not indicated.

^eFor Met codons AUG i and AUG e, i and e stand for initiator and elongator tRNA^Met, respectively.