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. 2008 May;179(1):177–192. doi: 10.1534/genetics.108.089128

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Figure 5.— — Purification and identification of endogenous free CrSUMO96 from C. reinhardtii after boiling of cell extracts. Proteins in unboiled C. reinhardtii cell lysates (lanes 2 and 5) and boiled lysates at 1× (lanes 3 and 6) and 3× (lanes 4 and 7) loading concentrations were detected by Coomassie blue staining (lanes 1–4) or with anti-CrSUMO96 antiserum on immunoblots of the SDS–PAGE (lanes 5–7). The asterisk (*) indicates the endogenous free CrSUMO96. Molecular size markers are shown at the left (lane 1).