TABLE 2.

Cleavage and binding domains of the C. reinhardtii SUMO proteins, their chromosomal localization, and their relative abundance in RT–PCR experiments (see also Figure 3)

C. reinhardtii SUMO name	Patterns			N-term. ext.b	Nuclear localization	Chr. no.			Relative transcript abundancec
		GG	GN				ΨKXEa					Start	End
CrSUMO89A		IGNd		6	Nuclear lamina	17	2,511,141	2,510,908	+
CrSUMO89B		VGNd		19	Nuclear lamina	17	2,507,181	2,506,915	ND
CrSUMO90		VGNd		19	Nucleoplasm	17	2,503,656	2,503,420	++
CrSUMO96	QVGG		VKTE	20	Nucleoplasm	1	9,064,900	9,063,630	+++
CrSUMO97	QVGGG		VKSE	23	Nucleoplasm	1	9,068,532	9,065,061	—
CrSUMO148	QEGG (4) SRGG		VKAE	14	Nuclear lamina	16	1,920,521	1,917,872	++

N-term. ext., N-terminal extension; Chr. no., chromosome number.

^aOnly the canonical ΨKXE motif [a large aliphatic (isoleucine, valine, or leucine), a lysine, any residue, followed by a glutamic or aspartic acid residue] was found. These proteins lack the alternative TKXE, TKED, AKCP, VKYC, and VKFT motifs.

^bThe number of residues amino terminal to the ubiquitin domain.

^cData are provided in Figure 3.

^dDoes not contain double glycine motif.