Figure 2.—

Timing of CLN1 gene transcription and new bud formation in α-factor synchronized bar1Δ and yta7Δ bar1Δ cells and in nocodazole synchronized wild-type and yta7Δ cells. Cells were blocked with α-factor (A and B) or nocodazole (C and D), released, and samples were taken at the indicated times. For each time point, the amount of late G₁-cyclin CLN1 transcript relative to ACT1 transcript was determined by real-time PCR (A and C). Error bars are the standard deviation. The formation of new buds was also monitored (B and D).