Figure 4.—

Nocodazole blocking alters the observed yta7Δ transcriptional defect in histone gene regulation. (A) Wild-type (solid circles) and yta7Δ (open squares) cells were blocked with nocodazole and released. Samples were taken at the indicated times and analyzed as in Figure 3. Darkly shaded regions emphasize the increased histone gene transcription upon gene deletion of YTA7. Error bars are the standard deviation. (B) HTB1 gene transcription is upregulated upon deletion of HIR2. Wild-type (solid circles) and hir2Δ (open squares) cells were blocked with nocodazole and released. Samples were taken at the indicated times and analyzed as in Figure 3. Error bars are the standard deviation. Asterisks show significant differences determined by one-tailed t-testing (P < 0.05).