Figure 5.—

FACS analysis of DNA content for bar1Δ and bar1Δyta7Δ or wild-type and yta7Δ cells. (A) bar1Δ and bar1Δyta7Δ cells were blocked with α-factor, released, and samples were taken at the indicated times for FACS analysis. (B) Histograms of FACS data from A showing 1N and 2N DNA contents as a percentage of gated cells. (C) Wild-type and yta7Δ cells were blocked with nocodazole, released, and samples were taken at the indicated times for FACS analysis. The asterisks highlight the key differences between the WT and yta7Δ cells. (D) Histograms of FACS data from C showing 1N and 2N DNA contents as a percentage of gated cells.