Figure 8.—

Yta7 does not direct or correlate with general H3K56 acetylation. (A) H3K56ac is not dependent on Yta7. ChIP to histone H3K56ac was performed in wild-type, yta7Δ, and H3K56R cells. Real-time PCR was used to measure enrichment at the intergenic region between HTA1 and HTB1 relative to ACT1. ChIP to unmodified H3 was used to control for nucleosome occupancy. Error bars are the standard deviation of the mean. The asterisk shows the significant difference determined by t-testing (P < 0.01). (B) Genomewide Yta7 binding does not correlate with H3K56ac. Over the 177 sites identified from ChIP-chip analysis of Yta7, only 6.8% were overlapping with previously reported H3K56ac-binding sites (Xu et al. 2005).