Figure 1.—

Loss of Ccr4 function results in abnormally large cells. The mean cell sizes of diploid wild-type, ccr4Δ, cln3Δ, or bck2Δ cells grown in YPD as described were plotted as a function of cell number (A). Average mean cell sizes were 92 ± 1 fl (WT), 106 ± 4 fl (ccr4Δ), 110 ± 5 fl (bck2Δ), and 128 ± 10 fl (cln3Δ). Coulter counter plots of quiescent wild-type cells from YPD cultures grown continuously in a shaking 30° incubator for 5 days demonstrate that cells from saturated cultures are smaller (50 ± 4 fl) than proliferating log-phase cells (92 ± 1 fl). However, similarly derived ccr4Δ cells were considerably larger (62 ± 3 fl) (B). Coulter counter plots of mid-log-phase cells from YPD grown cultures indicate that ccr4Δ cells have cell size plots that resemble bck2Δ cells (C).