TABLE 3.
Genetic interactions assessed by impact on mean cell size
| Cell size (fl) ±SD
|
||
|---|---|---|
| Gene deletions | Single deletion | +ccr4Δ |
| ccr4Δ/ccr4Δ | 106 ± 4 | |
| bck2Δ/bck2Δ | 110 ± 5 | 107 ± 5 |
| cln1Δ/cln1Δ | 95 ± 7 | 101 ± 8 |
| cln2Δ/cln2Δ | 90 ± 4 | 112 ± 8 |
| cln3Δ/cln3Δ | 128 ± 10 | Inviablea |
| mbp1Δ/mbp1Δ | 96 ± 3 | 93 ± 3 |
| swi4Δ/swi4Δ | 96 ± 6 | 111 ± 2 |
| swi6Δ/swi6Δ | 99 ± 5 | Inviableb |
| cln1Δ cln2Δ/cln1Δ cln2Δ | 135 ± 15 | Inviablec |
To examine genetic interactions, diploid cells with the indicated gene deletions were created and mid-log-phase YPD-grown cells were sized. Averages are the result of multiple independent measurements. SD, standard deviation.
a
From the dissection of 126 tetrads (100 from +/cln3Δ∷LEU2+/whi5Δ∷NAT +/bck2Δ∷kanmx∷URA3 +/ccr4Δ:KanMX heterozygotes and 26 from +/ccr4Δ:KanMX +/cln3Δ:KanMX heterozygotes), no viable ccr4Δcln3Δ spores were obtained.
b
A total of 49 tetrads were dissected and no viable ccr4Δswi6Δ spores were obtained.
c
A total of 40 tetrads were dissected and no viable ccr4Δcln1Δcln2Δ spores were obtained.