Figure 2.—

Ectopic dpp expression is due to overexpression of two microRNAs, miR-12 and miR-283. dpp expression is visualized with the dpp-lacZ reporter gene (red) in wing imaginal discs. Clones of cells driving expression of PUAS-29B3 (A and B), PUAS-miR12 alone (E and F), or PUAS-miR12 together with PUAS-miR283 (C and D), are labeled with GFP (green). Ectopic dppZ expression (arrows) is observed in more anterior clones following overexpression of PUAS-29B3, two copies of miR-12, or one copy each of miR-12 and miR-283. Note that ectopic dpp expression is mostly observed at the anterior edge of the wing pouch, as if this region is more sensitive to the activation of the Hh pathway. We also note that the expression of Dpp in the clones is variable, likely due to tissue deformation in the outgrowth.