Figure 5.—

An absence of the microRNA cluster does not lead to Hh phenotype but changes the level of the sensor constructs. (A–H) Clones of cells homozygous mutant for Δ3miR are marked by the absence of Myc (red). Cos2 (A–A′), Fu (B–B′), Smo (C–C′), Ci (D–D′) and dGMAP (H–H′) proteins are visualized by immunofluorescence (green). GFP expression of Cos2- (E–E′), Fu- (F–F′) and Smo- (G–G′) sensor constructs is in green. No modifications in the levels of the proteins (A–D) were observed except for dGMAP (H). In contrast GFP level of the sensor constructs modestly increased (E–G, arrows) in Δ3miR mutant clones.