Figure 6.—

Absence of Dcr-1 function changes Cos2- and Smo-sensor level without affecting their protein levels. (A–E) Clones of cells homozygous mutant for dcr-1 are marked by the absence of β-Gal (red). Cos2 (A–A″), Fu (B–B″), and Smo (C–C″) proteins are visualized by immunofluorescence (green). No modifications in the levels of the proteins were observed. GFP expression of miR12 (D–D″), Cos2- (E–E″), and Smo- (F–F″) sensor lines is in green. Note that GFP level strongly increases in dcr-1 mutant clones. Note also that, although dcr-1 mutant clones have been induced in L1, as attested by the big size of the wt twin spots (bright red), they reach a small size only likely due to cell lethality. Due to technical difficulties we could not analyze Fu-sensor constructs in dcr-1 mutant clones.