TABLE 2.
C. reinhardtii | C. r. protein ID | A. thaliana | A. t. localization | A. t. AGI gene model | E. coli | Synechocystis | S. cerevisiae | Molecular functionor protein domaina | |
---|---|---|---|---|---|---|---|---|---|
ISC | NFS1 | 118253 | NFS1 | Mit | At5g65720 | IscS | Slr0387 | Nfs1 | Cysteine desulfurase |
ISD11 | 184200 | ISD11 | Mitb | At5g61220 | Isd11 | Unknown | |||
ISU1 | 187899 | ISU1, -2, -3 | Mit | At4g22220, At3g01020 At4g04080 | IscU | Isu1, -2 | Fe–S scaffold | ||
ISCA1 | 196772 | Not annotated | Mitb | At2g16710 | IscA | Isa1, -2 | Fe–S scaffold | ||
IBA57c | 187731d | Not annotated | Mitb | At4g12130 | Unknown | ||||
NFU2 | 205851e 205852e | NFU4, NFU5 | Mit | At3g20970, At1g51390 | NP_312283 | Nfu1 | Fe–S scaffold | ||
MFDX | 154720 | ADX1, -2 = MFDX1, -2 | Mit | At4g05450 | Fdx | Yah1 | Ferredoxin | ||
ARH1 | 134840 | ADXR = MFDR | Mit | At4g32360 | Arh1 | Reductase | |||
MNP1 | 133548 | HCF101-L1 | Mitf | At4g19540 | Mrp | P-loop NTPasea | |||
Not specialized?g | Not specialized?g | HscA | Ssq1 | Chaperone (HSP70) | |||||
HSC20 | 144583 | Not annotated | Mitb | At5g06410 | HscB | Jac1 | Cochaperone | ||
MGE1 | 132633 | Not annotated | Mitb | At5g55200 | Mge1 | Nucleotide release factor | |||
FTX1 | 196778 | FH | Mit | At4g03240 | CyaY | Yfh1 | Iron chaperone | ||
GRX5 | 195767 | Not annotated | Mitb | At3g15660 | Grx | Grx5 | Glutaredoxin | ||
Export | CDS1h | 133289 | ATM1 | Mit | At4g28630 | Atm1 | Half ABC transporter | ||
ATM2 | 105113 | ATM2 | Mit | At4g28620 | Atm1 | Half ABC transporter | |||
ATM3 | 99736 | ATM3 = STA1 | Mit | At5g58270 | Atm1 | Half ABC transporter | |||
ERV1i | 114232 | ERV1 | Mit | At1g49880 | Erv1 | Sulfhydryl oxidase | |||
CIA | Absent | Absent | Mrp | Cfd1 | P-loop NTPasea | ||||
MNP2 | 206480 | HCF101-L2 | Cytosolf | At5g50960 | Nbp35 | P-loop NTPasea | |||
IOP1 (HYD3) | 206492d | NAR1 | Cytosolb | At4g16440 | Nar1 | Hydrogenase-likea | |||
CIA1 | 158085 | Not annotated | Cytosolb | At2g26060 | Cia1 | WD40 protein | |||
SUF | SUFA | 100292 | SUFA = ISCA | Plastid | At1g10500 | SufA | Slr1417 | Fe–S scaffold | |
SUFB | 132600d | SUFB = NAP1 | Plastid | At4g04770 | SufB | Slr0074 | ABC/ATPase | ||
SUFC | 289 | SUFC = NAP7 | Plastid | At3g10670 | SufC | Slr0075 | ABC/ATPase | ||
SUFD | 154386 | SUFD = NAP6 | Plastid | At1g32500 | SufD | Slr0076 | ABC/ATPase | ||
SUFE | 156669 | SUFE1, -2, -3 (N-term.)j | Plastid | At4g26500, At1g67810, At5g50210 | SufE | Slr1419 | Unknown | ||
SUFS | 206481 | NFS2 = CpNifS | Plastid | At1g08490 | SufS/CsdB | Slr0077 | Cysteine desulfurase | ||
NFU | NFU1 | 206476 | NFU1, -2, -3 | Plastid | At4g01940, At5g49940, At4g25910 | NP_312283 | Ssl2667 | Fe–S scaffold | |
NFU3 | 188996 | NFU1, -2, -3 | Plastid | Ssl2667 | Fe–S scaffold | ||||
HCF101 | HCF101 | 102098d | HCF101 | Plastid | At3g24430 | Slr0067 | DUF59 + P-loop NTPasea | ||
Hydrogenase maturation | HYDEF | 128256 | Absent | Radical SAM + GTPasea | |||||
HYDG | 196226 | Absent | Radical SAMa | ||||||
Other | CSD2 | 167884 | Absent | Sll0704 | Cysteine desulfurase | ||||
DFO1 | 206478 | Not annotated | At1g68310 | DUF59 (unknown)a | |||||
ISCA2 | 162474d | Not annotated | Mit/plastidb | At5g03905 | ErpA? | Fe–S scaffold |
Organisms: C. r., Chlamydomonas reinhardtii; A. t., the higher plant Arabidopsis thaliana; E. coli, the proteobacterium Escherichia coli; cyanobacterium Synechocystis sp. PCC 6803; and S. cerevisiae, Baker's yeast, Saccharomyces cerevisiae.
Function inferred from conserved protein domain(s). DUF59, domain of unknown function 59 (Pfam PF01883); radical SAM, radical S-adenosylmethionine enzyme.
Predicted.
IBA57, iron–sulfur cluster assembly factor for biotin synthase- and aconitase-like mitochondrial proteins, Mr 57 kDa. (Gelling et al. 2008).
The sequence is incomplete or not deemed correct because of annotation issues.
The two models are generated by alternative splicing, which appears to be conserved in Volvox. The significance of this remains to be investigated.
K. Bych and J. Balk, unpublished results.
The Hsp70 proteins HscA from E. coli and Ssq1 from yeast are specialized for Fe–S cluster assembly, but do not share the same genetic origin. The yeast Yarrowia lipolytica does not have a specialized Hsp70, and this situation may be the case for most eukaryotes (Schilke et al. 2006).
CDS1, Cadmium sensitive 1, is the only Chlamydomonas gene characterized experimentally (Hanikenne et al. 2005).
Two genes with high similarity to ERV1 are present in Chlamydomonas. This may reflect the situation in S. cerevisiae, where ERV1 is a sulfhydryl oxidase of the mitochondrial inner membrane space, whereas ERV2 resides in the endoplasmic reticulum.
In Arabidopsis, SUFE3 is a fusion protein of NADA and a SUFE domain (Murthy et al. 2007).