jar322/Df(3R)S87-5 adults do not express the myosin VI protein in any tissue. Western blot analysis of carcasses, ovaries, and testes in a (A) zygotic null myosin VI mutant, (B) maternal and zygotic null myosin VI mutant, and (C) zygotic null myosin VI mutant that expresses a myosin VI cDNA transgene. One-half of a whole fly, two ovaries, and two testes were loaded except for the mutant expressing the myosin VI transgene (one-quarter of an ovary).