Figure 4.

Immunological Quantification of Lhc Proteins in Thylakoid Membranes of Wild-Type and Mutant Plants.

Lhc proteins of purified thylakoid membranes were immunodetected with specific antibodies. The mean optical density of bands developed in four lanes (loaded with 1.0, 0.75, 0.50, and 0.25 μg of chlorophyll, respectively) was plotted against amount of chlorophyll loaded to assess the linearity of response and compared with the optical density of reaction bands of an antibody directed to the PSII core subunit CP47. Total amount of each subunit is expressed as a percentage of the corresponding wild-type content. Data are expressed as means ± sd (n = 4). Significantly different values from wild-type membranes are marked with an asterisk (according to Student's t test, P < 0.05).