Table 1.
Photosynthetic Pigment Content of the Wild Type and Mutants
| Wild Type | koCP24 | koCP26 | koCP24/26 | ||
|---|---|---|---|---|---|
| Chlorophyll (mg cm−2) | 16.8 ± 1.0 | 16.4 ± 0.6 | 15.9 ± 1.7 | 14.3 ± 0.8* | |
| Chlorophyll a/b | 3.04 ± 0.06 | 3.20 ± 0.10 | 3.06 ± 0.10 | 2.96 ± 0.09 | |
| Chlorophyll/carotenoid | 3.29 ± 0.04 | 3.23 ± 0.05 | 3.19 ± 0.13 | 3.18 ± 0.16 | |
| Dark-Adapted Leaves | Neo | 4.7 ± 0.2 | 4.9 ± 0.1 | 4.8 ± 0.1 | 5.1 ± 0.2 |
| Viola | 3.9 ± 0.9 | 4.1 ± 0.2 | 3.6 ± 0.1 | 4.0 ± 0.1 | |
| Anthera | – | – | – | – | |
| Lute | 14.5 ± 0.5 | 14.4 ± 0.3 | 15.4 ± 0.4 | 15.7 ± 1.0 | |
| β-Carotenoid | 7.1 ± 0.1 | 7.3 ± 0.1 | 7.2 ± 0.1 | 6.3 ± 0.5 | |
| Light-Treated Leaves | Viola | 1.4 ± 0.1 | 2.1 ± 0.5 | 1.3 ± 0.1 | 1.4 ± 0.1 |
| Anthera | 1.0 ± 0.1 | 1.3 ± 0.1 | 0.8 ± 0.1 | 1.2 ± 0.1 | |
| Zea | 1.2 ± 0.1 | 0.7 ± 0.2* | 1.3 ± 0.1 | 0.9 ± 0.1 | |
| (Z + 0.5A)/(V + A + Z) | 0.47 ± 0.04 | 0.32 ± 0.08* | 0.51 ± 0.06 | 0.42 ± 0.05 |
Pigment content is expressed as mol/100 mol chlorophylls. Viola, antheraxanthin, and Zea content was determined after leaves were illuminated for 30 min at 1000 μmol m−2 s−1. The (Z+1/2A)/(Z+A+V) ratio quantifies the operation of the xanthophyll cycle. Data are mean values of four experiments. Significantly different values with respect to the wild type are marked with an asterisk (P > 0.05).