Table 1.

Viability and Moisture Content in Wild-Type and ATHK1 Mutant Seeds

	Ws	athk1-3	athk1-4	athk1/ATHK1	35S:ATHK1
Survival	7.9% ± 1.9%	3.6% ± 2.0%	3.7% ± 2.8%	8.5% ± 3.2%	13.1% ± 2.8%
Moisture	12.5% ± 2.5%	15.4% ± 2.0%	14.2% ± 1.5%	10.4% ± 2.1%	9.0% ± 2.3%

For seed survival tests, seeds were subjected to 80% humidity and 60°C for 4 d as a CD treatment. Each value represents the mean percentage of germination for four replicates of at least 100 seeds. For seed moisture tests, ∼200 mg of seeds were dried at 65°C for 16 h, and seed mass before and after drying was measured. Measurements from four independently harvested seed lots were averaged. Error indicated is se. Measurements from athk1-3, athk1-4, and 35S:ATHK1 are significantly different from those of the wild type (P < 0.01, based on two-tailed t test).