Figure 2.

Characterization of the Δitr1 Δino1 Yeast Double Mutant Expressing the INT1 cDNA in the Sense (SSY36) or Antisense (SSY37) Orientation.

(A) Growth of SSY36 and SSY37 on minimal media supplemented with the indicated concentrations of myo-inositol.

(B) Subcellular localization of INT1-GFP in SSY16 cells. The images show yeast cells with one or more vacuoles that were photographed either under GFP excitation light (confocal section; left) or under white light (Nomarski section; right). Vacuoles of some cells are marked with arrows. Bar = 5 μm.

(C) Ion chromatographic analyses of myo-inositol concentrations determined in whole-cell ethanol extracts of SSY1 (1), SSY9 (9), SSY16 (16), SSY36 (36), and SSY37 (37) cells that were grown in liquid medium with 10 μg/mL myo-inositol and harvested either during the logarithmic growth phase or in the stationary phase. The genotypes of the different yeast strains are shown in the inset.