Table 2.

Effects of Avr1b Mutations on PCD Suppression and Interaction with Rps1b

					Rps1b Interaction
	PCD Suppressionb				Particle Bombardmentc		P. sojae Transformantsd
	Direct		Indirect		Rps1b to rps		No. of Transformants	Phenotype
Mutanta	Ratio to Control	P Value	Ratio to Control	P Value	Ratio	P Value		Rps1b	rps
Avr1b+	3.36	<0.001	2.84	<0.001	0.22	<0.001	2	A	V
Avr1bP7076	0.74	>0.5	0.74	>0.1	1.01	>0.1	2	V	V
Avr1bK1	2.41e	<0.001	4.87e	<0.001	0.12	<0.001	nt
Avr1bW1	0.99	>0.4	0.68	>0.1	1.02	>0.1	4	V	V
Avr1bW2	3.48	<0.001	3.81	<0.001	0.93	<0.001	4	V	V
Avr1bW3	0.85	>0.5	0.67	>0.1	1.01	>0.1	nt
Avr1bW4	1.03	>0.2	0.82	>0.1	1.05	>0.1	nt
Avr1bW5	0.93	>0.5	1.06	>0.1	0.17	<0.001	nt
Avr1bW6	3.12	<0.001	3.46	<0.001	0.18	<0.001	nt
Avr1bY1	1.80f	<0.01	1.55g	<0.05	1.00	>0.1	2	V	V

^aSequences of Avr1b mutant proteins given in Figure 6.

^bPCD suppression was measured using both the direct and indirect assays as described in the Methods and in the legend of Table 1. Significant deviation above 1.0 indicates activity in PCD suppression.

^cA significant reduction in GUS expression on Rps1b leaves compared with rps leaves indicates a positive interaction with Rps1b.

^dTwo to four independent transformants carrying each tested mutant Avr1b-1 gene were inoculated onto soybean Rps1b or rps seedlings to determine virulence (V) or avirulence (A). To test the P7076 allele, strain P7076 itself was transformed with a GUS gene. More detailed data are presented in Supplemental Table 4 online. nt, not tested.

^eNot significantly different than wild-type Avr1b (P > 0.100).

^fSignificantly different than wild-type Avr1b (P < 0.025).

^gSignificantly different than wild-type Avr1b (P < 0.001).