Figure 2.

Structure of PEN2 and PEN2-4 Genes.

(A) Structure of the PEN2 gene and location of the mutation in exon 5 in the pen2-4 gene from coi1-16 (marked by an asterisk). The G-to-A transition results in the elimination of an AciI site. Below are shown the positions of the AciI restriction sites in a 706-bp fragment amplified from PEN2, pen2-1, and pen2-4.

(B) Restriction of the 706-bp fragment from PEN2 (amplified using the primers 5′-AAACGTTGCCGTTGATTTCT-3′and 5′-CAGCAACACTAGCGCCATTA-3′) from gl1, pen2-1, coi1-16, and coi1-1 plants with AciI. The letters “a” and “b” indicate two different lines of coi1-16.