Figure 6.

Production of AVR2 and Inhibition in AF.

(A) Recombinant AVR2 triggers hypersensitive cell death in tomato leaves of MM-Cf2 plants. Recombinant AVR2 was injected into leaves of MM-Cf2 and MM-Cf2/rcr3-3 plants. Picture was taken 4 d after injection.

(B) Inhibition of PLCPs in AFs by AVR2. AF of BTH-treated tomato plants were preincubated with 66 nM AVR2 before adding DCG-04 to label the remaining noninhibited proteases. Biotinylated proteins were detected using streptavidin-HRP. A representative of three independent experiments is shown.

(C) Fluorescent protease activity profiling of PLCPs in AFs in presence or absence of AVR2. AFs of BTH-treated tomato plants were preincubated with 83 nM AVR2 before adding TMR-DCG-04 to label the remaining noninhibited proteases. Proteins were separated by 2D gel electrophoresis, and fluorescent proteins were visualized. A representative of three independent experiments is shown.