Abstract
The wild-type strain and four DNA repair-deficient strains (uvrA6, uvrB5, recA56, and polA1) of Escherichia coli K-12 were treated with acetic acid, lactic acid, and p-aminobenzoic acid at pH 3.5 during their stationary phase of growth. All three acids were highly toxic to the polymerase-deficient strain. The greater sensitivity of the strain carrying the polA1 gene than its isogenic pol+ derivatives suggested that damage caused by acidity requires polA+ gene products for repair.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Amsden A. B., Small D. K., Gomez R. F. Complex medium toxicity to some DNA repair-deficient strains of Salmonella typhimurium. Can J Microbiol. 1977 Oct;23(10):1494–1496. doi: 10.1139/m77-221. [DOI] [PubMed] [Google Scholar]
- Ananthaswamy H. N., Eisenstark A. Repair of hydrogen peroxide-induced single-strand breaks in Escherichia coli deoxyribonucleic acid. J Bacteriol. 1977 Apr;130(1):187–191. doi: 10.1128/jb.130.1.187-191.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bielecki J., Hrebenda J., Kwiatkowski Z. Mutants of Escherichia coli K12 sensitive to acidic pH. J Gen Microbiol. 1982 Aug;128(8):1731–1733. doi: 10.1099/00221287-128-8-1731. [DOI] [PubMed] [Google Scholar]
- Hunter D. R., Segel I. H. Effect of weak acids on amino acid transport by Penicillium chrysogenum: evidence for a proton or charge gradient as the driving force. J Bacteriol. 1973 Mar;113(3):1184–1192. doi: 10.1128/jb.113.3.1184-1192.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hurst A. Bacterial injury: a review. Can J Microbiol. 1977 Aug;23(8):935–944. doi: 10.1139/m77-139. [DOI] [PubMed] [Google Scholar]
- Przybylski K. S., Witter L. D. Injury and recovery of Escherichia coli after sublethal acidification. Appl Environ Microbiol. 1979 Feb;37(2):261–265. doi: 10.1128/aem.37.2.261-265.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rosenkranz H. S., Carr H. S., Morgan C. Unusual growth properties of a bacterial strain lacking DNA polymerase. Biochem Biophys Res Commun. 1971 Aug 6;44(3):546–549. doi: 10.1016/s0006-291x(71)80117-1. [DOI] [PubMed] [Google Scholar]
- Roth L. A., Keenan D. Acid injury of Escherichia coli. Can J Microbiol. 1971 Aug;17(8):1005–1008. doi: 10.1139/m71-160. [DOI] [PubMed] [Google Scholar]