Figure 4.

The Trichome Actin Cytoskeleton Defects of the scar2;scar4 and the scar1 scar2;scar3;scar4 Mutants Are Indistinguishable from Strong wave and arp2/3 Mutants.

The left panels are the maximum projections of confocal image stacks of whole-mount trichomes probed with phalloidin. Boxes in the left panels show regions highlighted in adjacent panels to the right. The right panels are the projections of branch midplanes that contain core cytoplasmic bundles. Bars = 5 μm.

(A) and (B) F-actin organization in a wild-type stage 4 trichome showing actin bundles that are aligned with the long axis of trichome branch elongation.

(C) and (D) F-actin organization in a scar2;scar4 stage 4 trichome showing disorganized actin filaments or bundles.

(E) and (F) F-actin labeling of a similarly staged scar1 scar2;scar3;scar4 quadruple mutant.

(G) and (H) F-actin labeling of a brk1 stage 4 trichome.

(I) and (J) F-actin organization in an arpc2 (dis2) stage 4 trichome.