Figure 6.

Quantitative Analysis of SCAR Transcript Levels in Wild-Type and scar2 Trichomes.

Steady state mRNA levels of SCAR genes were quantified in trichomes purified from wild-type and scar2 plants (see Methods). mRNA levels were quantified by qRT-PCR, and SCAR expression was normalized to the GAPC (AT3G04120) gene. The normalized transcript values represent the means ± se of triplicate measurements as described in Methods. Groups with an identical superscripted letter were not significantly different according to an ANOVA Tukey multiple comparison test (P < 0.01). The scar2 (dis3-1) allele causes premature transcriptional termination after the SHD-encoding exons (Basu et al., 2005).