Table 4.
Quantitative Analysis of the Epidermal Pavement Cell Shape Phenotype of scar, brk1, and dis2 Mutants
| Genotype | Circularitya | Skeleton Endsb | Area (× 104 μm2) |
|---|---|---|---|
| Col | 0.20 ± 0.01 (30) ab | 12.1 ± 0.5 (n = 30) ab | 2.02 ± 0.07 (30) ab |
| scar1 | 0.21 ± 0.01 (30) ab | 12.0 ± 0.6 (n = 30) abc | 2.24 ± 0.11 (30) a |
| scar2 | 0.19 ± 0.01 (30) ab | 11.5 ± 0.5 (n = 30) bc | 1.59 ± 0.07 (30) cde |
| scar3 | 0.17 ± 0.01 (30) a | 14.0 ± 0.7 (n = 30) a | 1.84 ± 0.13 (30) abcde |
| scar4 | 0.18 ± 0.01 (30) ab | 12.3 ± 0.5 (n = 30) ab | 1.85 ± 0.08 (30) abcde |
| scar1 scar2 | 0.22 ± 0.01 (30) abc | 10.1 ± 0.5 (n = 30) bcd | 1.56 ± 0.07 (30) e |
| scar2;scar3 | 0.23 ± 0.01 (30) bc | 10.6 ± 0.4 (n = 30) bc | 1.52 ± 0.06 (30) ef |
| scar2;scar4 | 0.23 ± 0.01 (30) bc | 9.4 ± 0.4 (n = 30) cde | 2.00 ± 0.10 (30) abc |
| scar1 scar2;scar3 | 0.27 ± 0.01 (30) cd | 8.0 ± 0.4 (n = 30) de | 1.98 ± 0.08 (30) abcd |
| scar1;scar3;scar4 | 0.22 ± 0.01 (30) ab | 10.6 ± 0.4 (n = 30) bc | 1.99 ± 0.08 (30) abc |
| scar1 scar2;scar3;scar4 | 0.30 ± 0.01 (30) d | 7.8 ± 0.4 (n = 30) e | 1.57 ± 0.06 (30) de |
| brk1-2 | 0.29 ± 0.02 (30) d | 7.5 ± 0.4 (n = 30) e | 1.61 ± 0.10 (30) cde |
| dis2-1(arpc2) | 0.30 ± 0.01 (30) d | 7.5 ± 0.4 (n = 30) e | 1.65 ± 0.09 (30) bcde |
| brk1-2 er-105 | ND | ND | 1.14 ± 0.07 (20) f |
a
Circularity is a descriptor of shape complexity and is calculated as 4 π(area)/(perimeter)2. Data are mean values ± se. Numbers in the parentheses indicate the sample size. The letters indicate ANOVA Tukey multiple comparison test groupings. Mean values with the same letter in the group are not significantly different (α = 0.05). ND, not determined.
b
Number of skeleton end points calculated from the medial-axis transformation of thresholded individual pavement cell images.