Table 5.
The Sequence of the Oligonucleotide Primers Used in This Work
| Name | Sequence |
|---|---|
| Primers for the characterization of scar1-t1 | |
| SALK_017554-F | 5′-GGAATGAAATGGGAGAAATGA-3′ |
| SALK_017554-R | 5′-CGTGAAATTGTAAGAACTAAGAAGCA-3′ |
| Primers for the characterization of scar3-t1 | |
| SALK_036994-F | 5′-TCACTCCAGGAAGACATTTGC-3′ |
| SALK_036994-R | 5′-AAAACTGTTGGCCATAAGCC-3′ |
| Primers for the characterization of scar4-t1 and scar4-t2 | |
| SALK_116410-F | 5′-TCTTTCTCCTGACGCGTTTT-3′ |
| SALK_116410-R | 5′-TGAATCCTCAGGCGTCTCTT-3′ |
| SALK_022766-F | 5′-TCACTCCAGGAAGACATTTGC-3′ |
| SALK_022766-R | 5′-AAAACTGTTGGCCATAAGCC-3′ |
| Primers for the characterization of scarl-t1 | |
| SALK_055413-F | 5′-TGAACTAAAAGCTTGTCCCTTACCG-3′ |
| SALK_055413-R | 5′-CGTCAGTTAGGCGATCTCGCTG-3′ |
| T-LB | 5′-CCGTCTCACTGGTGAAAAGAA-3′ |
| RT-PCR primers | |
| SCAR1SHD-F | 5′-CACCAGAATTCACATGTATGACGAAT-3′ |
| SCAR1SHD-R | 5′-CTCGAGGTTTAGCCACAGAGGCAAGTCTTG-3′ |
| SCAR1WA-F | 5′-CACCAGAATTCTCCAAAATCCAGCTGAGCC-3′ |
| SCAR1WA-R | 5′-CGGCTCGAGTCATGTGTCGCTATCGC-3′ |
| SCAR3SHD-F | 5′-CACCATGGAATGCCACGGAATGTATACGG-3′ |
| SCAR3SHD-R | 5′- GAATTCGGCCAAACGTGACATATCTCGAC-3′ |
| SCAR3WA-F | 5′-CACCGAATTCCTCCAAAATCCTGCTGAGCCC-3′ |
| SCAR3WA-R | 5′-CTCGAGTTACGTTCACTCCATGTATCGC-3′ |
| SCAR4SHD-F | 5′-CACCGAATTCATGGCATTGACGAGATACCAG-3′ |
| SCAR4SHD-R | 5′-CTCGAGCGTCTCTTTAATAGTTGTGTGAG-3′ |
| SCAR4WA-F | 5′-CACCATGGTATCCCCTGAGTCCATAC-3′ |
| SCAR4WA-R | 5′-GCGGATCCTCACTCGCTCCAGCTATCTG-3′ |
| SCAR4C_F2 | 5′-CACCATGGTATCCCCTGAGTCCATAC-3′ |
| SCAR4C_R2: | 5′-GCGGATCCTCACTCGCTCCAGCTATCTG-3′ |
| qRT-PCR primers | |
| SCAR1QPCR-F | 5′-AAATGTCTCTTACCTCCAAAATCCA-3′ |
| SCAR1QPCR-R | 5′-TTCGAAACCATCCACTGTAACG-3′ |
| SCAR2QPCR-F | 5′-TGACCCTGAAGCTCTGCTTGA-3′ |
| SCAR2QPCR-R | 5′-CAGCGAGATCACCGAGTTGA-3′ |
| SCAR3QPCR-F | 5′-CTTCTTGCAACAAATCCGAACA-3′ |
| SCAR3QPCR-R | 5′-GCAGTAGCCGATGATGTCGTT-3′ |
| SCAR4QPCR-F | 5′-TGGTCTCGTCGGCGTTTT-3′ |
| SCAR4QPCR-R | 5′-CATGCAGGCAATGGAATACCT-3′ |
| GAPCQPCR-F | 5′-TCCCGTGTGGTCGACTTGA-3′ |
| GAPCQPCR-R | 5′-CCACTCCCTATCATTCGAGATCTG-3′ |
| GL2QPCR-F | 5′-TGTCAATGGCCGTCGACATGTCTT-3′ |
| GL2QPCR-R | 5′-TTGATCTGTGTGCGGCGGTTTT-3′ |
| Primers for the cloning of WA domains | |
| SCAR3WA-F | 5′-CACCGGATCCTCCTTGTCAAATTCTGAGCCGTGG-3′ |
| SCAR3WA-R | 5′-GTCGACTTACGTATCACTCCATGTATCGCTTTCAT-3′ |
| SCAR4WA-F | 5′-CACCGGATCCAACATCCAGAGGGATGAAAACACACA-3′ |
| SCAR4WA-R | 5′-GTCGACTCACTCGCTCCAGCTATCTG-3′ |